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Glaucoma is a group of eye diseases characterized by the thinning of the retinal nerve fiber layer (RNFL), which is primarily caused by the progressive death of retinal ganglion cells (RGCs). Precise monitoring of these changes at a cellular resolution in living eyes is significant for glaucoma research. In this study, we aimed to assess the effectiveness of temporal speckle averaging optical coherence tomography (TSA-OCT) and dynamic OCT (dOCT) in examining the static and potential dynamic properties of RGCs and RNFL in living mouse eyes. We evaluated parameters such as RNFL thickness and possible dynamics, as well as compared the ganglion cell layer (GCL) soma density obtained fromin vivoOCT, fluorescence scanning laser ophthalmoscopy (SLO), andex vivohistology.

 

Extreme cold events over North America such as the February 2021 cold wave have been suggested to be linked to stratospheric polar vortex stretching. However, it is not resolved how robustly and on which timescales the stratosphere contributes to the surface anomalies. Here we introduce a simple measure of stratospheric wave activity for reanalyses and model outputs. In contrast to the well-known surface influences of sudden stratospheric warmings (SSWs) that increase the intraseasonal persistence of weather regimes, we show that extreme stratospheric wave events are accompanied by intraseasonal fluctuations between warm and cold spells over North America in observations and climate models. Particularly, strong stratospheric wave events are followed by an increased risk of cold extremes over North America 5–25 days later. Idealized simulations in an atmospheric model with a well-resolved stratosphere corroborate that strong stratospheric wave activity precedes North American cold spells through vertical wave coupling. These findings potentially benefit the predictability of high-impact winter cold extremes over North America.

 

Cold winters over Eurasia often coincide with warm winters in the Arctic, which has become known as the “warm Arctic–cold Eurasia” pattern. The extent to which this observed correlation is indicative of a causal response to sea ice loss is debated. Here, using large multimodel ensembles of coordinated experiments, we find that the Eurasian temperature response to Arctic sea ice loss is weak compared to internal variability and is not robust across climate models. We show that Eurasian cooling is driven by tropospheric and stratospheric circulation changes in response to sea ice loss but is counteracted by tropospheric thermodynamical warming, as the local warming induced by sea ice loss spreads into the midlatitudes by eddy advection. Although opposing effects of thermodynamical warming and dynamical cooling are found robustly across different models or different sea ice perturbations, their net effect varies in sign and magnitude across the models, resulting in diverse model temperature responses over Eurasia. The contributions from both tropospheric dynamics and thermodynamics show substantial intermodel spread. Although some of this spread in the Eurasian winter temperature response to sea ice loss may stem from model uncertainty, even with several hundred ensemble members, it is challenging to isolate model differences in the forced response from internal variability.

 

Optical coherence tomography (OCT) and scanning laser ophthalmoscopy (SLO) are imaging technologies invented in the 1980s that have revolutionized the field ofin vivoretinal diagnostics and are now commonly used in ophthalmology clinics as well as in vision science research. Adaptive optics (AO) technology enables high-fidelity correction of ocular aberrations, resulting in improved resolution and sensitivity for both SLO and OCT systems. The potential of gathering multi-modal cellular-resolution information in a single instrument is of great interest to the ophthalmic imaging community. Although similar instruments have been developed for imaging the human retina, developing such a system for mice will benefit basic science research and should help with further dissemination of AO technology. Here, we present our work integrating OCT into an existing mouse retinal AO-SLO system, resulting in a multi-modal AO-enhanced imaging system of the living mouse eye. The new system allows either independent or simultaneous data acquisition of AO-SLO and AO-OCT, depending on the requirements of specific scientific experiments. The system allows a data acquisition speed of 200 kHz A-scans/pixel rate for OCT and SLO, respectively. It offers ∼6 µm axial resolution for AO-OCT and a ∼1 µm lateral resolution for AO-SLO-OCT imaging.

 

Abstract While there is substantial evidence for tropospheric jet shift and Hadley cell expansion in response to greenhouse gas increases, quantitative assessments of individual mechanisms and feedback for atmospheric circulation changes remain lacking. We present a new forcing-feedback analysis on circulation response to increasing CO 2 concentration in an aquaplanet atmospheric model. This forcing-feedback framework explicitly identifies a direct zonal wind response by holding the zonal mean zonal wind exerting on the zonal advection of eddies unchanged, in comparison with the additional feedback induced by the direct response in zonal mean zonal wind. It is shown that the zonal advection feedback accounts for nearly half of the changes to the eddy-driven jet shift and Hadley cell expansion, largely contributing to the subtropical precipitation decline, when the CO 2 concentration varies over a range of climates. The direct response in temperature displays the well-known tropospheric warming pattern to CO2 increases, but the feedback exhibits negative signals. The direct response in eddies is characterized by a reduction in upward wave propagation and a poleward shift of midlatitude eddy momentum flux (EMF) convergence, likely due to an increase in static stability from moist thermodynamic adjustment. In contrast, the feedback features a dipole pattern in EMF that further shifts and strengthens midlatitude EMF convergence, resulting from the upper-level zonal wind increase seen in the direct response. Interestingly, the direct response produces an increase in eddy kinetic energy (EKE), but the feedback weakens EKE. Thus, the forcing-feedback framework highlights the distinct effect of zonal mean advecting wind from direct thermodynamic effects in atmospheric response to greenhouse gas increases.